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In this work, a methodology for building and calibrating the kinetic scheme for the 1D CFD model of a zone-coated automotive Diesel Oxidation Catalyst (DOC) by means of a Genetic Algorithm (GA) approach is presented. The methodology consists of a preliminary experimental activity followed by a modelling, optimization and validation process. The tested aftertreatment component presents zone coating, with the front brick side covered with Zeolites in order to ensure hydrocarbons trapping at low temperature, and Platinum Group Metal (PGM), while the rear brick side presents an alumina washcoat with a different PGM loading. Reactor scale samples representative of each coating zone were tested on a Synthetic Gas Bench (SGB), to fully characterize the component’s behavior in terms of Light-off and hydrocarbons (HC) storage for a wide range of inlet feed compositions and temperatures, representative of engine-out conditions.