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Recent pressures on vehicle manufacturers to reduce their average fleet levels of CO2 emissions have resulted in an increased drive to improve fuel economy and enable use of fuels developed from renewable sources that can achieve a net reduction in the CO2 output of each vehicle. The most popular choice for spark-ignition engines has been the blending of ethanol with gasoline, where the ethanol is derived either from agricultural or cellulosic sources such as sugar cane, corn or decomposed plant matter. However, other fuels, such as butanol, have also arisen as potential candidates due to their similarities to gasoline, e.g. higher energy density than ethanol. To extract the maximum benefits from these new fuels through optimized engine design and calibration, an understanding of the behaviour of these fuels in modern engines is necessary.