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A prototype catalyst has been developed and integrated within the aftertreatment exhaust system to control the HC, CO, PM and NOx emissions from diesel exhaust gas. The catalyst activity in removing HC and nano-particles was examined with exhaust gas from a diesel engine operating on biodiesel - Rapeseed Methyl Ester (RME). The tests were carried out at steady-state conditions for short periods of time, thus catalyst tolerance to sulphur was not examined. The prototype catalyst reduced the amount of hydrocarbons (HC) and the total PM. The quantity of particulate with electrical mobility diameter in nucleation mode size < 10nm, was significantly reduced over the catalyst. Moreover, it was observed that the use of EGR (20% vol.) for the biodiesel fuelled engine significantly increases the particle concentration in the accumulation mode with simultaneous reduction in the particle concentration in the nuclei mode.