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The objective of this study was to evaluate iso-butanol (C4H9OH) as an alternative fuel for spark ignition engines. Unlike methanol (CH3OH) and ethanol (C2H5OH), iso-butanol has not been extensively studied in the past as either a fuel blend candidate with gasoline or straight fuel. The performance of a single cylinder engine (ASTM=CFR) was studied using alcohol-gasoline blends under different input parameters. The engine operating conditions were: three carburetor settings (three different fuel flow rates), spark timings of 5°, 10°, 15°, 20°, and 25° BTDC, and a range of compression ratios from a minimum of 7.5 to a maximum of 15 in steps of one depending on knock. The fuels tested were alcohol-gasoline blends having 5%, 10%, 15%, and 20% of iso-butanol, ethanol, and methanol. And also as a baseline fuel, pure gasoline (93 ON) was used. The engine was run at a constant speed of 800 RPM.