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Operation of flex fuel vehicles requires operation with a range of fuel properties. The significant differences in the heat of vaporization and energy density of E0-E100 fuels and the effect on spray development need to be fully comprehended when developing engine control strategies. Limited enthalpy for fuel vaporization needs to be accounted for when developing injection strategies for cold start, homogeneous and stratified operation. Spray imaging of multi-hole gasoline injectors with fuels ranging from E0 to E100 and environmental conditions that represent engine operating points from ambient cold start to hot conditions was performed in a spray chamber. Schlieren visualization technique was used to characterize the sprays and the results were compared with Laser Mie scattering and Back-lighting technique. Open chamber experiments were utilized to provide input and validation of a CFD model.