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Controlling ignition delay is the key to successfully enable partially premixed combustion in diesel engines. This paper presents experimental results of partially premixed combustion in an optically accessible engine, using primary reference fuels in combination with artificial exhaust gas recirculation. By changing the fuel composition and oxygen concentration, the ignition delay is changed. To determine the position of the flame front, high-speed visualization of OH-chemiluminescence is used, enabling a cycle-resolved analysis of OH formation. A clear correlation is observed between ignition delay and flame location. The mixing of fuel and air during the ignition delay period defines the local equivalence ratio, which is estimated based on a spherical combustion volume for each spray. The corresponding emission measurements using fast-response analyzers of CO, HC and NOX confirm the decrease in local equivalence ratio as a function of ignition delay.