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A well-balanced use of alternative fuels worldwide is an important objective for a sustainable development of individual transportation. Several countries have objectives to substitute a part of the energy of traffic by ethanol as the renewable energy source. The global share of Bioethanol used for transportation is continuously increasing. Investigations of limited and unregulated emissions of a flex fuel vehicle with gasoline-ethanol blend fuel have been performed in the present work on the chassis dynamometer according to the measuring procedures, which were established in the previous research in the Swiss Network to adequately consider the transient (WLTC) and the stationary operation (SSC). The investigated fuel contained ethanol (E), in the portions of 10% & 85% by volume. The investigated vehicle represented a newer state of technology and an emission level of Euro 5. The engine works with homogenous GDI concept and with 3-W-catalyst (3WC).