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AASHTO I-Beam is a standard structural concrete part for bridge sections. The flexural performance of an AASHTO I-Beam is critical for bridge design. This paper presents an application of Digital Image Correlation (DIC) Method in full-scale AASHTO I-Beam flexural performance study. A full-scale AASHTO I-Beam pre-stressed with steel strands is tested by three-point bending method. The full-scale AASHTO I-Beam is first loaded from 0 kips to 100 kips and is then released from 100 kips to 0 kips. A dual-camera 3D Digital Image Correlation (DIC) system is used to measure the deflection and strain distribution during the testing. From the DIC results, the micro-crack generation progress during the loading progress can be observed clearly from the measured DIC strain map. To enable such a large-scale DIC measurement, the used DIC setup is optimized in terms of the optical imaging system and speckle pattern size.