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This paper reviews the development and testing of the distillation subsystem of water regeneration system from urine (WRS-UM) based on a method of vacuum distillation with a rotary multistage vacuum distiller and a thermal pump. Test results show that with relatively small power consumption the subsystem using rotary three-stage vacuum distiller provides high rates of heat and mass transfer processes, useful productivity and distillate quality. The conducted tests have confirmed that it will be efficient to use the presented system as a part of WRS-UM system in Russian segment of the International Space Station.