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Biological pre-treatment of early planetary/lunar base wastewater has been extensively studied because of its predicted ability to offer equivalent system mass (ESM) savings for long term space habitation. Numerous biological systems and reactor types have been developed and tested for treatment of the generally unique waste streams associated with space exploration. In general, all systems have been designed to reduce organic carbon (OC) and convert organic nitrogen (ON) to nitrate and/or nitrite (NOx -). Some systems have also included removal of the oxidized N in order to reduce overall oxygen consumption and produce additional N2 gas for cabin use. Removal of organic carbon will generally reduce biofouling as well as reduce energy and consumable cost for physiochemical processors.

Microbial control in closed environmental systems, such as those of spacecraft or proposed base missions is typically limited to disinfection in the potable water system by a strong chemical agent such as iodine or chlorine. However, biofilm growth in the environmental system tubing threatens both the sterility of the potable water distribution as well as operational problems with wastewater systems. In terrestrial systems, biofilm has been recognized for its difficulty to control and eliminate as well as resulting operational problems. In order to maintain a potable water source for crew members as well as preventing operational problems in non-sterile systems, biofilm needs to be considered during system design. While biofilm controls can limit biofilm buildup, they are typically disruptive and cannot completely eliminate biofilm. Selenium coatings have shown to prevent initial biofilm attachment as well as limit attached growth on a variety of materials.

Hollow fiber membrane reactors (HFMBRs) may be used for biological wastewater treatment, and may be integrated with NASA's current research developments. The goal of this paper is to (a) evaluate the effect of mass transfer and hydrodynamics in a microporous HFMBR and (b) appropriateness of HFMBRs for use in space applications. Even though bubble-less aeration was not achieved by the use of microporous membranes, mass transfer within the HFMBR was found to increase after biofilm formation. Conversely, convective flow dominated transport within the system. Despite the high treatment efficiency obtained by the HFMBR, due to the bioreactor size, configuration and membrane spacing within the HFMBR, the bioreactor was not a suitable option for application under microgravity conditions. Even though developing a system with more favorable system hydrodynamics would aid in treatment efficiency, the use of a microporous HFMBR is not a recommended option to meet NASA's needs.

The feasibility of a long-term space mission is partially reliant upon the ability to effectively recycle wastewater. Merged biological and physiochemical processes (integrated water recovery systems (IWRS)) are capable of producing potable water at lower equivalent system mass (ESM) than treatment systems composed of only physiochemical processes. Reducing the ESM of the water recycling units can increase the practicality of extended space missions by decreasing payload weight. In order to lower the ESM of the biological pre-treatment component, a single-stage biological reactor capable of simultaneous carbon and nitrogen removal was created by modifying the membrane-aerated biofilm reactor (MABR) design. Studies were performed in order to evaluate the water quality performance of this reactor.

Hollow fiber membranes aerate wastewater without bubble formation by separating the liquid and gases phases with a semi-permeable membrane. These membranes have shown to successfully create aerobic conditions within a biological reactor. This research investigated the effect of long term usage and biofilm growth on membrane's ability to transfer oxygen to solution. Results show that oxygen transfer across the membrane decreased significantly compared to unused membranes in areas of high biofilm growth while low biofilm growth showed only slight decreases.

This research is based on the hypothesis that recycling biofilm can provide the required carbon to increase biological denitrification of the carbon limited early planetary base wastewater. Recycling biofilm may offer significant advantages including a reduction in solid waste from biological wastewater processors, increased N2 return to cabin air, a reduction in TDS loading to the RO system, and increased alkalinity to drive further nitrification. The results of the study indicate that denitrification rate did increase due to the addition of lysed biofilm derived from the nitrification reactor. However, there was a simultaneous large release of additional ammonium. Further work will be required to understand the magnitude of the ammonium release and overall benefits of the process.

The purpose of this study was to evaluate the viability of treating wastewater through edible plant hydroponics. After the harvest in the hydroponic experiment (32 day study period), plant yield for edible biomass (corresponds to the harvested leaves) in wastewater and hydrosol (control) were 0.131 kg/m2 and 0.104 kg/m2, respectively. Potassium, TDS, and TN showed decreasing trends in hydrosol and wastewater during the experiment. Nitrification was observed in the wastewater unit with a significant increase (92.5%) in nitrate mass. Nitrite and ammonium mass in wastewater decreased with time, while hydrosol had negligible amounts of nitrite and ammonium during the study period. Calcium and magnesium masses decreased in the control and increased in wastewater. Wastewater showed a decrease in the mass of TOC (19.7%), while the hydrosol had negligible mass with respect to TOC.

From 1997 to 2001, the third author worked with a team of engineers at JSC to develop the requirements and basic design for the Bioregenerative Planetary Life Support Systems Test Complex, or BIO-Plex. Under the Advanced Integration Matrix (AIM) Project, this earlier effort is extended to an investigation of methods and approaches for Advanced Systems Integration and Control. The intent is to understand and validate the use of software as an integrating function for complex heterogeneous systems, particularly for Advanced Life Support (ALS), in the context of support of mission operations. Preliminary investigations undertaken in the summer of 2004 indicate that integration of controls for the type of dynamic, non-linear, closed-loop biological systems under investigation for ALS systems require a different systems engineering approach than that required for traditional avionics systems.

Texas colonias are unincorporated subdivisions characterized by inadequate water and wastewater infrastructure, inadequate drainage and road infrastructure, substandard housing, and poverty. Since 1989 the Texas Legislature has implemented policies to halt further development of colonias and to address water and wastewater infrastructure needs in existing and new colonias along the border with Mexico. Government programs and non-government and private organization projects aim to address these infrastructure needs. Texas Tech University's Water Resources Center demonstrated the use of alternative on-site wastewater treatment in the Green Valley Farms colonia, San Benito, Texas. The work in Green Valley Farms was a component of a NASA-funded project entitled “Evaluation of NASA's Advanced Life Support Integrated Water Recovery System for Non-Optimal Conditions and Terrestrial Applications.” Two households within the colonia were demonstration sites for the constructed wetlands.

The objective of this study was to investigate the potential of membrane-aerated bioreactors as long term microgravity compatible nitrifying biological water processors (BWP). A small-scale (1/20th) replica of the water recovery system (WRS) at JSC has been operated and extensively analyzed at Texas Tech University (TTU) for the last 3 years. The current nitrifying tubular reactor at JSC and TTU has experienced difficulty in maintaining efficiency and low maintenance. In an attempt to increase the efficiency of the biological portion of the WRS, a membrane-aerated bioreactor (MABR) was constructed and operated using the same parameters as the TTU-WRS in August 2003. The MABR is downstream of an anaerobic packed bed and is designed to promote nitrification (NH4 → NOx). The MABR achieved a percent nitrification of 61% and 55% for recycle ratios of 10 and 20, respectively.

Simulated wastewater, known as early surface mission wastewater, treated in previous experiments at JSC and TTU included urinal flush water, shower water, humidity condensate, oral hygiene water, and hand wash water. In reality, there is a difference between the early surface mission wastewater and the International Space Station wastewater. The ISS does not have a shower or hand wash, which contributes approximately 59 percent of the make-up water treated. The average influent ammonia concentration in the simulated wastewater treated by the TTU water reclamation system frequently exceeds 500 mg/L. Removal of the shower make-up water in simulated wastewater will result in a significant increase in the ammonia concentration, resulting in higher influent pH values and ammonia concentrations that may be inhibitory. Biological treatment technologies have suitably treated the diluted waste stream but a more concentrated waste stream may present a greater challenge.

Biological pre-treatment of liquid waste could potentially offer equivalent mass savings for long term space habitation. Previous work has demonstrated the technological feasibility. However, limited work has been conducted on optimizing the biological reactors or fully characterizing the biochemical transformations occurring within the reactors. The objective of these studies was to provide long-term operating data on a proposed and well studied reactor configuration, and explore the effects of RR on system performance. The water recovery system has been in successful operation for over 2 years. Data to be presented will include both typical removal efficiencies for nitrogen species, DOC as well as important water quality parameters. In addition the effect of recycle ratio (2X, 5X, 10X, and 20X) will be quantified.

For prolonged manned spaceflight, recycling of wastewater is critical to minimize payload costs. We have constructed a pilot-scale, closed-loop water recycling system (CLWRS). Due to slow process dynamics, evaluation of multiple experimental scenarios is very time-consuming. To accelerate evaluation, we have developed mathematical models of the individual reactors, as well as a process model of the pilot plant, which combines nitrification, denitrification, recycle, and degassing steps. The simulation accurately reproduces the 35% total nitrogen (TN) reduction observed experimentally at a 20/1 recycle ratio. Both experiments and simulations indicate that biological CLWRS have significant potential for long-duration manned space flight.

Bioreactor studies and microcosm experiments were conducted to determine the degradation potential of a commercial cleansing formulation. With the possible replacement of the current cleansing formulation under consideration (Ecolab whole body shampoo containing Igepon TC-42™ as an active ingredient), determination of the degradation characteristics of the alternative formulation is necessary. The commercial formulation currently being evaluated is a modified version of Pert Plus® for Kids (PPK). The degradation potential of the PPK and main surfactant Sodium Laureth Sulfate (SLES) was determined in a packed bed denitrifying bioreactor. Results from the bioreactor studies led to the development of stoichiometric relationships to help predict and monitor SLES degradation. In addition to the degradation rates of Ecolab, the PPK formulation, as well as the four leading constituents contained in the PPK formulation was determined under denitrifying conditions in microcosm studies.

When compared to physical and chemical processes for wastewater treatment in space, the benefits of biological systems include reduced storage and handling of waste material, lower energy requirements and plant growth system compatibility. An advanced membrane reactor (AMR) was constructed to treat ammonium-rich simulated wastewater. The effluent pH was approximately 6.3, and ammonium and TOC reduction rates were greater than 60 percent and 99 percent, respectively. The experimental results demonstrate that this technology may be suitable for space applications. However, the long-term performance of these systems should be investigated.

This research compared the nutrient content of the Biological Water Processor (BWP) effluent at JSC with acceptable nutrient ranges for general hydroponic NFT-solutions. Evaluated nutrient-components were NO3-N, P, K, Ca, Mg, Fe, Mn, Zn, B, Cu and Mo. Compared to Cooper's and Molyneaux's solution (Jones, 1997) BWP-nutrient concentrations were low for Ca, Mg, Fe and B. Compared to the NFT-solution used at KSC (Wheeler et al., 1997), the BWP-effluent showed higher contents of P, K, Zn, Cu and Mo and lower contents of N, Ca, Mg, Fe and B. This indicates that the BWP-effluent could support NFT-plant production.