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A new method to determine simultaneously the temperature and the fuel vapor concentration inside an evaporating spray was described by using a laser-induced fluorescence technique. A TMPD doped base fuel composed of C12H26: 22%, C13H28: 54% and C14H30: 30% was injected into the combustion chamber of a rapid compression machine which is filled with a high temperature and high pressure nitrogen. The laser sheet was used for incident light, which was reflected by a prism located inside the combustion chamber and propagated through the center of an evaporating spray. The laser induced fluorescence intensity was imaged by a high speed-gated intensifier from a direction perpendicular to the incident light. The results shows that mixtures with high equivalence ratio are observed in the central region, while low equivalence ratio mixtures are observed in the periphery of the spray. It is also observed that the temperature of richest mixture is 50 K as low as the surrounding gas temperature.