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This paper summarizes the first phase of an experimental effort focused on developing a comprehensive understanding of the in-cylinder air/fuel mixing and combustion processes in spark-ignition engines using laser-based fuel distribution and combustion measurements. As part of this first phase, a semi-quantitative, laser-induced fluorescence, fuel distribution measurement technique was developed and demonstrated. The calibration, correction, and image analysis processes associated with the technique were shown to be comparatively simple and effective (relative to other analytical and empirical methods). The error associated with the technique was shown to be 5 - 10 % under vapor phase conditions. This work was applied to a port fuel injected optical engine, which was designed for optical access through the piston and cylinder liner under firing conditions.