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Homogeneous Charge Compression Ignition (HCCI) combustion technology has demonstrated a profound potential to decrease both emissions and fuel consumption. In this way, the significance of the 2-stroke HCCI engine has been underestimated as it can provide more power stroke in comparison to a 4-stroke engine. Moreover, the mass of trapped residual gases is much larger in a 2-stroke engine, causing higher initial charge temperatures, which leads to easier auto-ignition. For controlling 2-stroke HCCI engines, it is vital to find optimized simulation approaches of HCCI combustion with a focus on ignition timing. In this study, a Computational Fluid Dynamic (CFD) model for a 2-stroke gasoline engine was developed coupled to a semi-detailed chemical mechanism of iso-octane to investigate the simulation capability of the considered chemical mechanism and the effects of different simulation parameters such as the turbulence model, grid density and time step size.