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Three replicate aerobic-heterotrophic biotrickling filters were designed to promote the simultaneous biodegradation of graywater and a waste gas containing NH3, H2S and CO2. Upon visual observation of discolored solids, it was originally hypothesized that gas-phase CO2 concentrations were excessive, causing regions of anoxic zones to form within the biotrickling filters. Observed discolored (black) biofilm of this nature is typically assumed to be either lysed bacterial cells or anaerobic regions, implying alteration of operational conditions. Solid (biofilm) samples were collected in the presence and absence of gas-phase wastestream(s) to determine if the gas-phase contaminants were contributing to the solid-phase discoloration. Two sets of experiments (shaker flask and solids characterization) were conduced to determine the nature of the discolored solids. Results indicated that the discolored solids were neither anaerobic bacteria nor lysed cells.

The ALS/NSCORT Education and Outreach provides an avenue to engage and educate higher education students and K-12 educators/students in the center's investigations of the synergistic concepts and principles required for regenerative life-support in extended-duration space exploration. The following K-12 Education programs will be addressed: 1) Key Learning Community Project provides exposure, mentoring and research opportunities for 9-12th grade students at Key Learning Community This program was expanded in 2004 to include an “Explore Mars” 3-day camp experience for 150 Key students. The overall goal of the collaborative project is to motivate students to pursue careers in science, technology, and engineering; 2) Mission to Mars Program introduces 5th-8th grade students to the complex issues involved with living on Mars, stressing the interdisciplinary fundamentals of science, technology and engineering that underlie Advanced Life Support research.

Complete reuse of graywater will be essential during long duration human space missions. The highest loaded and most important component to remove from graywater is surfactant, the active ingredient in soaps and detergents. When considering a biological treatment system for processing of graywater, surfactant biodegradability becomes a very important consideration. Surfactants should be chosen that are degraded at a fast rate and yield inconsequential degradation byproducts. Experiments conducted for this research examined the biodegradation of the surfactants in Pert Plus for Kids, disodium cocoamphodiacetate (DSCADA) and sodium laureth-3 sulfate (SLES), using respirometry. Rates of CO2 production, or ultimate degradation, are reported. DSCADA was found to be toxic to bacteria when present at 270 ppm whereas no toxicity was observed during experiments with SLES.

A detailed study was conducted on nitrification using a bench top bioprocessor system proposed for water recycling of a urine-soap wastewater expected to be generated by crewmembers on International Space Station (ISS) or similar long-term space missions. The bioprocessor system consisted of two packed bed biofilm reactors; one anoxic reactor used for denitrification and one aerobic reactor used for nitrification. lnfluent wastewater was a mixture of dilute NASA whole body soap (2,300 mg/L) and urea (500 mg/L as organic nitrogen). During two months of steady-state operation, average chemical oxygen demand (COD) removal was greater than 95%, and average total nitrogen removal was 70%. We observed that high levels of nitrite consistently accumulated in the aerobic (nitrifying) reactor effluent, indicating incomplete nitrification as the typical end product of the reaction would be nitrate.