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The Minus Eighty degrees Celsius Laboratory Freezer for ISS (MELFI) is a freezer that has been delivered to NASA as Laboratory Support Equipment (LSE) by ESA. One of the main concerns during the development of MELFI has been first the intrinsic thermal performance of the system, and, second, the thermal performance once on orbit. From the beginning the differences between the thermal performance on orbit and on ground were estimated through thermal analyses of the sub-systems to predict the behavior on orbit, and specific tests on ground simulating as far as possible a non-convective environment. The results were used to define the criteria for the performance to be obtained on ground, which would ensure the compliance with requirements on orbit. The complete verification campaign has demonstrated that the system is capable of giving the performance imposed by the criteria on ground for all mission aspects, including samples cooling down and protection during power-off.