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A novel microarray-based approach to simultaneous detection, quantitation, and identification of multiple microorganisms found in water and air supplies is presented. The rapid DNA/RNA probe-based approach avoids time consuming cell cultivation procedures by utilizing the inherent amplification property of rRNA combined with highly sensitive proximal CCD detection. Specifically, optimal DNA probes for simultaneously capturing Escherichia coli and Vibrio proteolyticus were arrayed in a 4 x 4 matrix using a novel microarray printer capable of depositing hundreds of probes within a single well (1cm2) of a conventional 96 well microtiter plate. Following hybridization of a 16S rRNA microorganism mixture to the DNA microarray, detection, imaging, quantitation and identification was performed within seconds employing the complimentary PC-based proximal CCD detector/imager.

Semi-solid metal (SSM) casting has long been identified as a high-volume process for producing safety-critical and structural automotive castings, but cost and complexity issues have limited its widespread commercial acceptance. Rheocasting, an SSM process that creates semi-solid slurry directly from liquid metal, eliminates the cost disadvantages of the process. However, the majority of rheocasting processes are complex and difficult to operate in the foundry environment. Recent work at MIT has led to the fundamental discovery that application of heat removal and convection as a molten alloy cools through the liquidus creates a non-dendritic, semi-solid slurry. A new process based on this understanding, S.S.R.™ (Semi-Solid Rheocasting), simplifies the rheocasting process by controlling heat removal and convection of an alloy during cooling using an external device. Solution heat treatable castings have been produced in a horizontal die casting machine with the S.S.R.™ process.

Compression in the head-neck complex often causes fractures of the first cervical vertebra (C1). The type of fracture often determines whether the injury is stable or unstable, which significantly affects the ultimate injury severity. The often unstable bursting fracture of C1 is thought to be caused by a transformation of axial compressive forces into lateral bursting forces by the wedge-shaped lateral masses. The purpose of this study was to measure the orientation of the joint surfaces of C1 to determine whether this symmetry exists. An additional goal was to determine whether the orientation of the joint surfaces varied significantly with location on the surface. Direct measurements of surface coordinates were taken from 40 dried vertebrae. The angles of two areas on each of the four joint surfaces of the lateral masses of C1 were then calculated. The resulting angles agreed with previous investigations of upper cervical vertebral anatomy.

The National Space Biomedical Research Institute (NSBRI), established in 1997, is a twelve-university consortium dedicated to research that will impact mankind's next exploratory steps. The NSBRI's Education and Public Outreach Program (EPOP), is supporting NASA's education mission to, “Inspire the next generations…as only NASA can,” through a comprehensive Kindergarten through post-doctoral education program. The goals of the EPOP are to: communicate space exploration biology to schools; support undergraduate and graduate space-based courses and degrees; fund postdoctoral fellows to pursue space life sciences research; and engage national and international audiences to promote understanding of how space exploration benefits people on Earth. NSBRI EPOP presents its accomplishments as an educational strategy for supporting science education reform, workforce development, and public outreach.