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Introduction of modern diesel aftertreatment, primarily selective catalytic reduction (SCR) designed to reduced NOx, has increased the presence of urea decomposition byproducts, mainly ammonia, in the aftertreatment system. This increase in ammonia has been shown to lead to particle formation in the aftertreatment system. In this study, a state of the art diesel exhaust fluid (DEF)-SCR system was investigated in order to determine the influence of DEF dosing on solid particle count. Post diesel particulate filter (DPF) particle count (> 23 nm) is shown to increase by over 400% during the World Harmonized Transient Cycle (WHTC) due to DEF dosing. This increase in tailpipe particle count warranted a detailed parametric study of DEF dosing parameters effect on tailpipe particle count. Global ammonia to NOx ratio, DEF droplet residence time, and SCR catalyst inlet temperature were found to be significant factors in post-DPF DEF based particle formation.