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The operation of a crop growth system in micro-gravity is an important part of the National Aeronautics and Space Administration's Closed Ecological Life Support System development program. Maintaining densely arrayed plants in a closed environment imposed to induce high growth rates must be expected to result in substantial levels of water transpiration rate. Since the environmental air is recirculated, the transpiration water must be removed. In an operating CELSS, it is expected that this water will provide potable water for use of the crew. There is already considerable knowledge about water removal from crew environmental air during orbital and transfer activities, and the difference between the conditions of the described requirement and the conditions for which experience has been gained is the quantities involved and the reliability implications due to the required periods of operation.

We present a device for collecting and storing feces in microgravity that is user-friendly yet suitable for spacecraft in which cabin volume and mass are constrained. On Apollo missions, the commode function was served using disposable plastic bags, which proved time-consuming and caused odor problems. On Skylab, the space shuttle, and the International Space Station, toilets have used airflow beneath a seat to control odors and collect feces. We propose to incorporate airflow into a system of self-compacting, self-drying collection and stowage bags, providing the benefits of previous commodes while minimizing mass and volume. Each collection bag consists of an inner layer of hydrophobic membrane that is permeable to air but not liquid or solid waste, an outer layer of impermeable plastic, and a collapsible spacer separating the inner and outer layers. Filled bags are connected to space vacuum, compacting and drying their contents.

The initial concepts and construction of a three layered, water-absorbent, zero-G, compactor trash bag will be described. This bag is composed of an inner wicking layer, a middle absorbent layer, and an outer containment layer. The primary properties of the wicking layer are the fast adsorption of any free liquid released within the trash bag and the lateral spreading of this liquid around the interior of the bag. The absorbent layer sequesters and stores the liquid captured by the wicking layer. It need not be as fast acting as the wicking layer, but has to have a much larger capacity. The containment layer allows for handling of the bag without worry of releasing the contents. The combined strength of the three layers needs to be sufficient to withstand the forces exerted by the compactor.

This study examines the current state of the art in rodent habitats as well as the next generation of rodent habitats currently under development at NASAs Ames Research Center. Space Shuttle missions are currently limited in duration to just over two weeks. In contrast to this, future life science missions aboard the Space Station may last months or even years. This will make resource conservation and utilization critical issues in the development of rodent habitats for extended microgravity missions. Emphasis is placed on defining rodent requirements for extended space flights of up to 90 days, and on improving habitability and extending the useful performance life of rodent habitats.

An innovative design that meets both Shuttle and Space Station requirements for a user-friendly, volume-efficient, portable glovebox system has been developed at Ames Research Center (ARC). The Standard Interface Glovebox (SIGB) has been designed as a two Middeck locker-sized system that mounts in a Middeck Rack Structure (MRS) or in any rack using the Standard Interface Rack (SIR) rail spacing. The MRS provides structural support for the SIGB during all aspects of the mission and is an interface consistent with NASA's desire for commonality of mechanical interfaces, allowing the SIGB to be flown on essentially any manned space platform. The SIGB provides an enclosed work volume which operates at negative pressure relative to ambient, as well as excellent lighting and ample work volume for anticipated life sciences-related experiment operations inflight.

The Flexible Membrane Commode (FMC) is an alternative waste management system designed to address the severe mass restrictions on the Orion vehicle. The concept includes a deployable seat and single use, three layer bags that employ air flow to draw solids away from the body and safely contain them in disposable bags.1 Simulated microgravity testing of the system was performed during two separate parabolic flight campaigns in July and August of 2008. Experimental objectives included verifying the waste fill procedures in reduced gravity, characterizing waste behavior during the filling process, and comparison of the results with model predictions. In addition the operational procedure for bag installation, removal, and sealing were assessed. 2 A difficult operational requirement concerns the delivery of the fecal waste simulant into the upper area of the bag in a manner that faithfully simulates human defecation.

Two separate experimental rigs used in tests on NASA and Zero-G Corporation aircrafts flying low-gravity trajectories, and in the NASA 2.2 Second Drop Tower have been developed to test the functioning of the Flexible Membrane Commode (FMC) concept under reduced gravity conditions. The first rig incorporates the flexible, optically opaque membrane bag and the second rig incorporates a transparent chamber with a funnel assembly for evacuation that approximates the size of the membrane bag. Different waste dispensers have been used including a caulking gun and flexible hose assembly, and an injection syringe. Waste separation mechanisms include a pair of wire cutters, an iris mechanism, as well as discrete slug injection. The experimental work is described in a companion paper. This paper focuses on the obtained results and analysis of the data.

The purification of waste water is a critical element of any long-duration space mission. The Vapor Phase Catalytic Ammonia Removal (VPCAR) system offers the promise of a technology requiring low quantities of expendable material that is suitable for exploration missions. NASA has funded an effort to produce an engineering development unit specifically targeted for integration into the NASA Johnson Space Center's Integrated Human Exploration Mission Simulation Facility (INTEGRITY) formally known in part as the Bioregenerative Planetary Life Support Test Complex (Bio-Plex) and the Advanced Water Recovery System Development Facility. The system includes a Wiped-Film Rotating-Disk (WFRD) evaporator redesigned with micro-gravity operation enhancements, which evaporates wastewater and produces water vapor with only volatile components as contaminants. Volatile contaminants, including organics and ammonia, are oxidized in a catalytic reactor while they are in the vapor phase.

Determining the fundamental role of gravity in vital biological systems in space is one of six science and research areas that provides the philosophical underpinning for why NASA exists. The study of cells, tissues, and microorganisms in a spaceflight environment holds the promise of answering multiple intriguing questions about how gravity affects living systems. To enable these studies, specimens must be maintained in an environment similar to that used in a laboratory. Cell culture studies under normal laboratory conditions involve maintaining a highly specialized environment with the necessary temperature, humidity control, nutrient, and gas exchange conditions. These same cell life support conditions must be provided by the International Space Station (ISS) Cell Culture Unit (CCU) in the unique environment of space. The CCU is a perfusion-based system that must function in microgravity, at unit gravity (1g) on earth, and from 0.1g up to 2g aboard the ISS centrifuge rotor.

The animal habitat under development for the International Space Station (ISS) provides a unique opportunity for the physiological and biological science community to perform controlled experiments in microgravity on rats and mice. This paper discusses the complexities that arise in developing a new animal habitat to be flown aboard the ISS. Such development is incremental and moves forward by employing the past successes, learning from experienced shortcomings, and utilizing the latest technologies. The standard vivarium cage on the ground can be a very simple construction, however the habitat required for rodents in microgravity on the ISS is extremely complex. This discussion presents an overview of the system requirements and focuses on the unique scientific and engineering considerations in the development of the controlled animal habitat parameters. In addition, the challenges to development, specific science, animal welfare, and engineering issues are covered.

This project is a Phase III SBIR contract between NASA and Water Reuse Technology (WRT). It covers the redesign, modification, and construction of the Wiped-Film Rotating-Disk (WFRD) evaporator for use in microgravity and its integration into a Vapor Phase Catalytic Ammonia Removal (VPCAR) system. VPCAR is a water processor technology for long duration space exploration applications. The system is designed as an engineering development unit specifically aimed at being integrated into NASA Johnson Space Center's Bioregenerative Planetary Life Support Test Complex (BIO-Plex). The WFRD evaporator and the compressor are being designed and built by WRT. The balance of the VPCAR system and the integrated package are being designed and built by Hamilton Sundstrand Space Systems International, Inc. (HSSSI) under a subcontract with WRT. This paper provides a description of the VPCAR technology and the advances that are being incorporated into the unit.

The General Purpose Work Station (GPWS) is a laboratory multi-use facility, as demonstrated during the Spacelab Life Sciences 1 (SLS-1) flight. The unit provided particulate containment under varying conditions, served as an effective work space for manipulating live animals, e.g., rats, served as a containment facility for fixatives, and was proposed for use to conduct in-flight maintenance during connector pin repair. The cabinet has a front door large enough to allow installation of a full-size microscope in-flight and is outfitted with a side window to allow delivery of items into the cabinet without exposure to the spacelab atmosphere. Additional support subsystems include inside cabinet mounting, surgical glove fine manipulations capability, and alternating or direct current power supply for experiment equipment, as will be demonstrated during Spacelab J.

The Vapor Phase Catalytic Ammonia Removal (VPCAR) system is being developed to recycle water for future NASA Exploration Missions. Testing was recently conducted on NASA's C-9B Reduced Gravity Aircraft to determine the microgravity performance of a key component of the VPCAR water recovery system. Six flights were conducted to evaluate the fluid dynamics of the Wiped-Film Rotating Disk (WFRD) distillation component of the VPCAR system in microgravity, focusing on the water delivery method. The experiments utilized a simplified system to study the process of forming a thin film on a disk similar to that in the evaporator section of VPCAR. Fluid issues are present with the current configuration, and the initial alternative configurations were only partial successful in microgravity operation. The underlying causes of these issues are understood, and new alternatives are being designed to rectify the problems.

Spacelab Life Sciences-3 (SLS-3) was scheduled to be the first United States man-tended microgravity flight containing Rhesus monkeys. The goal of this flight as in the five untended Russian COSMOS Bion flights and an earlier American Biosatellite flight, was to understand the biomedical and biological effects of a microgravity environment using the non-human primate as human surrogate. The SLS-3/Rhesus Project and COSMOS Primate-BIOS flights all utilized the rhesus monkey, Macaca mulatta. The ultimate objective of all flights with an animal surrogate has been to evaluate and understand biological mechanisms at both the system and cellular level, thus enabling rational effective countermeasures for future long duration human activity under microgravity conditions and enabling technical application to correction of common human physiological problems within earth's gravity, e.g., muscle strength and reloading, osteoporosis, immune deficiency diseases.

Life Sciences research on Space Station will utilize rats to study the effects of the microgravity environment on mammalian physiology and to develop countermeasures to those effects for the health and safety of the crew. The animals will produce metabolic water which must be reclaimed to minimize logistics support. The condensate from the Research Animal Holding Facility (RAHF) flown on Spacelab Life Sciences-2 (SLS-2) in October 1993 was used as an analog to determine the type and quantity of constituents which the Space Station (SS) water reclamation system will have to process. The most significant organics present in the condensate were 2-propanol, glycerol, ethylene glycol, 1,2-propanediol, acetic acid, acetone, total proteins, urea and caprolactam while the most significant inorganic was ammonia. Microbial isolates included Xanthomonas, Sphingobacterium, Pseudomonas, Penicillium, Aspergillus and Chrysosporium.

A multi-discipline, multi-year collaborative spaceflight research program (NASA/Mir Science Program) has been established between the United States and Russia utilizing the capabilities of the Russian Mir Space Station and the NASA space shuttle fleet. As a key research discipline to be carried out onboard Mir, fundamental biology research encompasses three basic objectives: first, to investigate long-term effects of microgravity upon plant and avian physiology and developmental biology; second, to investigate the long-term effects of microgravity upon circadian rhythm patterns of biological systems; and third, to characterize the long-term radiation environment (internal and external) of the Russian Mir space station. The first joint U.S./Russian fundamental biology research on-board Mir is scheduled to begin in March, 1995 with the Mir mission 18 and conclude with the docking of the U.S. shuttle to Mir in June, 1995 during the STS-71, Spacelab/Mir Mission-1 (SLM-1).

The Research Animal Holding Facility (RAHF) is a spaceflight-qualified hardware system for housing adult rats. The Neurolab Space Shuttle mission, targeted for February 1998 on STS-89, will include neuroscience experiments involving nursing rat dams and neonates (newborn rats). Rat neonates have never been previously flown for spaceflight experimentation, and they present unique life support, science, and engineering challenges in the Spacelab microgravity environment. Modifications of the RAHF (with an associated comprehensive testing program, including spaceflight) are currently underway at NASA Ames Research Center (ARC), in order to add to the RAHF the capability of supporting nursing dams and neonates in preparation for Neurolab.

Mixed liquid/solid wastes, including feces, water processor effluents, and food waste, can be lyophilized (freeze-dried) to recover the water they contain and stabilize the solids that remain. Our previous research has demonstrated the potential benefits of using thermoelectric heat pumps to build a lyophilizer for processing waste in microgravity. These results were used to build a working prototype suitable for ground-based human testing. This paper describes the prototype design and presents results of functional and performance tests.

Various methods have been used to simulate reduced gravity environments for space systems research and development. Neutral buoyancy has been the most universally used simulation of zero-g. This paper describes the facilities, personnel and experimental work that are associated with the Neutral Buoyancy Test Facility (NBTF) at NASA Ames Research Center (ARC). This facility provides a unique underwater environment for the researcher to simulate reduced gravity activities and evaluate the performance of space-related equipment. The NBTF's small size gives it several advantages over larger water facilities. First, a smaller crew ensures a lower overhead. Second, the facility is used for research purposes only, eliminating any scheduling conflicts with astronaut training. Lastly, the small volume of water allows the researcher to more easily vary the water temperature. This feature is ideal for investigations of astronaut thermal comfort and regulation.

Accurate root zone moisture control in microgravity plant growth systems is problematic. With gravity, excess water drains along a vertical gradient, and water recovery is easily accomplished. In microgravity, the distribution of water is less predictable and can easily lead to flooding, as well as anoxia. Microgravity water delivery systems range from solidified agar, water-saturated foams, soils and hydroponics soil surrogates including matrix-free porous tube delivery systems. Surface tension and wetting along the root substrate provides the means for adequate and uniform water distribution. Reliable active soil moisture sensors for an automated microgravity water delivery system currently do not exist. Surrogate parameters such as water delivery pressure have been less successful.