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This study reported black carbon (BC) mass and solid particle number emissions from a gasoline direct injection (GDI) vehicle and a port fuel injection (PFI) vehicle on splash blended E10 and iB16 fuels over the FTP-75 and US06 drive cycles at standard and cold ambient temperatures. For the FTP-75 drive cycle, the GDI vehicle had lower solid particle number and BC mass emissions from E10 (5.1×1012 particles/mile; 4.2 mg/mile) and iB16 (5.2×1012 particles/mile; 3.9 mg/mile) compared to E0 (7.2×1012 particles/mile; 7.0 mg/mi). Most of the reductions were attributed to the statistically significant reductions during the phases 1 and 2 of the FTP-75 drive cycle. iB16 was also observed to have statistically significant reduction on BC emissions when compared to E0 at cold ambient temperature but E10 did not show such BC reduction. For the PFI vehicle, most of the solid particle number and BC mass emissions were emitted primarily during phase 1 of the FTP-75 drive cycle.