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A habitat for housing up to 32 Tenebrionid, black body beetles (Trigonoscelis gigas Reitter) has been developed at Ames Research Center for conducting studies to evaluate the effects of long duration spaceflight upon insect circadian timing systems. This habitat, identified as the Beetle Kit, provides an automatically controlled lighting system and activity and temperature recording devices, as well as individual beetle enclosures. Each of the 32 enclosures in a Beetle Kit allows for ad lib movement of the beetle as well as ventilation of the beetle enclosure via an externally operated hand pump. Two Beetle Kits were launched on STS-84 (Shuttle-Mir Mission-06) on May 15, 1997 and were transferred to the Priroda module of the Russian Mir space station on May 18 as part of the NASA/Mir Phase 1 Science Program. Following the Progress collision with Spektr on June 25, the Kits were transferred to the Kristall module. The beetles will remain on Mir for approximately 135 days.

An innovative design that meets both Shuttle and Space Station requirements for a user-friendly, volume-efficient, portable glovebox system has been developed at Ames Research Center (ARC). The Standard Interface Glovebox (SIGB) has been designed as a two Middeck locker-sized system that mounts in a Middeck Rack Structure (MRS) or in any rack using the Standard Interface Rack (SIR) rail spacing. The MRS provides structural support for the SIGB during all aspects of the mission and is an interface consistent with NASA's desire for commonality of mechanical interfaces, allowing the SIGB to be flown on essentially any manned space platform. The SIGB provides an enclosed work volume which operates at negative pressure relative to ambient, as well as excellent lighting and ample work volume for anticipated life sciences-related experiment operations inflight.

Ames Research Center's Life Sciences Division was responsible for managing the development of fundamental biology flight experiments during the Phase 1 NASA/Mir Science Program. Beginning with astronaut Norm Thagard's historic March, 1995 Soyuz rendezvous with the Mir station and continuing through Andy Thomas' successful return from Mir onboard STS-91 in June, 1998, the NASA/Mir Science Program has provided scientists with unparalleled long duration research opportunities. In addition, the Phase 1 program has yielded many valuable lessons to program and project management personnel who are managing the development of future International Space Station payload elements. This paper summarizes several of the key space station challenges faced and associated lessons learned by the Ames Research Center Fundamental Biology Research Project.

The General Purpose Work Station (GPWS) is a laboratory multi-use facility, as demonstrated during the Spacelab Life Sciences 1 (SLS-1) flight. The unit provided particulate containment under varying conditions, served as an effective work space for manipulating live animals, e.g., rats, served as a containment facility for fixatives, and was proposed for use to conduct in-flight maintenance during connector pin repair. The cabinet has a front door large enough to allow installation of a full-size microscope in-flight and is outfitted with a side window to allow delivery of items into the cabinet without exposure to the spacelab atmosphere. Additional support subsystems include inside cabinet mounting, surgical glove fine manipulations capability, and alternating or direct current power supply for experiment equipment, as will be demonstrated during Spacelab J.

The Spacelab Life Sciences-2 (SLS-2) mission provided scientists with the unique opportunity of obtaining inflight rodent tissue and blood samples during a 14-day mission flown in October, 1993. In order to successfully obtain these samples, Ames Research Center's Space Life Sciences Payloads Office has developed an innovative, modular approach to packaging the instruments used to obtain and preserve the inflight tissue and blood samples associated with the hematology experiments on SLS-2. The design approach organized the multitude of instruments into 12 different 5x6x1 inch kits which were each used to accomplish a particular experiment functional objective on any given day during the mission. The twelve basic kits included blood processing, isotope and erythropoietin injection, body mass measurement, and microscope slides.

The Spacelab Life Sciences 2 (SLS-2) mission became NASA's longest duration Shuttle mission, lasting fourteen days, when Columbia landed on November 1, 1993. Located within the Spacelab were a total of 48 laboratory rats which were housed in two Research Animal Holding Facilities (RAHFs) developed by the Space Life Sciences Payloads Office (SLSPO) at Ames Research Center. In order to properly maintain the health and well-being of these important research animals, sufficient quantities of food and water had to be available for the duration of the mission. An Inflight Refill Unit was developed by the SLSPO to replenish the animals' drinking water inflight using the Shuttle potable water system in the middeck galley as the source of additional water. The Inflight Refill Unit consists of two major subsystems, a Fluid Pumping Unit (FPU) and a Collapsible Water Reservoir (CWR).

A multi-discipline, multi-year collaborative spaceflight research program (NASA/Mir Science Program) has been established between the United States and Russia utilizing the capabilities of the Russian Mir Space Station and the NASA space shuttle fleet. As a key research discipline to be carried out onboard Mir, fundamental biology research encompasses three basic objectives: first, to investigate long-term effects of microgravity upon plant and avian physiology and developmental biology; second, to investigate the long-term effects of microgravity upon circadian rhythm patterns of biological systems; and third, to characterize the long-term radiation environment (internal and external) of the Russian Mir space station. The first joint U.S./Russian fundamental biology research on-board Mir is scheduled to begin in March, 1995 with the Mir mission 18 and conclude with the docking of the U.S. shuttle to Mir in June, 1995 during the STS-71, Spacelab/Mir Mission-1 (SLM-1).

NASA is developing a Telescience Support Center (TSC) at the Ames Research Center. The center will be part of the infrastructure needed to conduct research in the Space Station and has been tailored to satisfy the requirements of the fundamental biology research program. The TSC will be developed from existing facilities at the Ames Research Center. Ground facility requirements have been derived from the TSC functional requirements. Most of the facility requirements will be satisfied with minor upgrades and modifications to existing buildings and laboratories. The major new development will be a modern data processing system. The TSC is being developed in three phases which correspond to deliveries of Biological Research Facility equipment to Station. The first phase will provide support for early hardware in flight Utilization Flight −1 (UF-1) in 2001.

The development of virtual environment technology at NASA Ames Research Center and other research institutions has created opportunities for enhancing human performance. The application of this technology to training astronaut flight crews planning to go onboard the International Space Station has already begun at the NASA Johnson Space Center. A unique application of virtual environments to crew training is envisioned at NASA Ames Research Center which combines state of the art technology with haptic feedback to create a method for training crewmembers on critical life sciences operations which require fine motor skills. This paper describes such a concept, known as the Virtual Glovebox, as well as surveys other applications of virtual environments to astronaut crew training.

Ames Research Center’s Life Sciences Division has developed and flown an extensive array of spaceflight experiment unique equipment (EUE) during the last decade of the twentieth century. Over this ten year span, the EUE developed at ARC supported a vital gravitational biology flight research program executed on several different platforms, including the Space Shuttle, Spacelab, and Space Station Mir. This paper highlights some of the key EUE elements developed at ARC and flown during the period 1990-1999. Resulting lessons learned will be presented that can be applied to the development of similar equipment for the International Space Station.