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Renewable fuels are essential in the field of heavy duty transportation if we are to reach a fossil-free society in the foreseeable future. However renewable diesel fuels based on fatty acid methyl ester (FAME) might face problems with degradation and with cold flow properties. From the perspective of an engine, this may cause problems in the fuel injection system, such as fuel filter clogging and injector deposits. These phenomena, especially fuel filter clogging, can be connected to gel-like soft particles, which could originate from degradation products as well as from byproducts created during biodiesel refining. In this study, soft particles from the degradation of bio-based diesel fuel were examined. The tested fuels included hydrogenated vegetable oils (HVO), rapeseed methyl ester (RME) and 10% blend of rapeseed methyl ester with standard diesel (B10).