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The Plant Generic BioProcessing Apparatus (PGBA), a plant growth facility developed for commercial space biotechnology research, has flown successfully on 3 spaceflight missions for 4, 10 and 16 days. The environmental control systems of this plant growth chamber (28 liter/0.075 m2) provide atmospheric, thermal, and humidity control, as well as lighting and nutrient supply. Typical performance profiles of water transpiration and dehumidification, carbon dioxide absorption (photosynthesis) and respiration rates in the PGBA unit (on orbit and ground) are presented. Data were collected on single and mixed crops. Design options and considerations for the different sub-systems are compared with those of similar hardware.