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The present study explores the effect of in-cylinder generated non-thermal plasma on hydroxyl and soot development. Plasma was generated using a newly developed Microwave Discharge Igniter (MDI), a device which operates based on the principle of microwave resonation and has the potential to accentuate the formation of active radical pools as well as suppress soot formation while stimulating soot oxidation. Three diagnostic techniques were employed in a single-cylinder small-bore optical diesel engine, including chemiluminescence imaging of electronically excited hydroxyl (OH*), planar laser induced fluorescence imaging of OH (OH-PLIF) and planar laser induced incandescence (PLII) imaging of soot. While investigating the behaviour of MDI discharge under engine motoring conditions, it was found that plasma-induced OH* signal size and intensity increased with higher in-cylinder pressures albeit with shorter lifetime and lower breakdown consistency.