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High-resolution planar laser-induced fluorescence (PLIF) measurements were performed on the scalar field in an optical engine. The measurements were of sufficient resolution to fully resolve all of the length scales of the flow field through the full cycle. The scalar dissipation spectrum was calculated, and by fitting the results to a model turbulent spectrum the Batchelor scale of the turbulent flow was estimated. The scalar inhomogeneity was introduced by a low-momentum gas jet injection. A consistent trend was observed in all data; the Batchelor scale showed a minimum value at top dead center (TDC) and was nearly symmetric about TDC. Increasing the engine speed resulted in a decrease of the Batchelor scale, and the presence of a shroud on the intake valve, which increased the turbulence intensity, also reduced the Batchelor scale. The effect of the shrouded valve was less significant compared to the effect of engine speed.