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Soot emission, known as PM (particulate matter), is becoming a big issue for GDI engines as the emission regulations being increasingly stricter. It is found that ethanol, as an oxygenated bio-fuel, can reduce the soot emission when added to gasoline. In order to fully understand the effect of ethanol on soot reducing, the soot characteristics of ethanol/gasoline blends were studied on laminar diffusion flames. In this experiment, the blending ratio of ethanol/gasoline was set as E0/20/40/60/80. Considering the carbon content decreasing due to ethanol addition, carbon mass flow rate was remained constant. The two-dimensional distributions of soot volume fraction were measured quantitatively by using two-color laser induced incandescence technique. The results showed that ethanol is able to decrease the soot significantly, but the effect of ethanol on soot reduction is weakened with the increasing ethanol ratio.