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This study was set out to characterize particulate emissions from diesel engines in terms of poly aromatic hydrocarbon emissions and Benzene Soluble Organic Fraction. The characteristics of DPM vary with engine operating conditions, quality of fuel and lubricants being used. Hence the diesel exhaust for the purpose of toxicity characterization needs to be studied for Organic Matter in terms of Poly Aromatic Hydrocarbon (PAH) and Benzene Soluble Fraction (BSF). Therefore, the objectives of the present research are to characterize the diesel exhaust particulate matter for the above parameters under varying engine operating conditions/loads. Six PAHs, namely Chrysene, Benzo (k) Flouranthene, Benzo (a) Pyrene, Dibenzo (a, h) Anthracene, Benzo (g,h,i) Perylene and Indenopyrene were analyzed on High Pressure Liquid Chromatography (HPLC). PAH concentrations in the particulates of Mahindra DI engine were affected by engine loads.