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The use of Artificial Neural Networks (ANNs) as a predictive tool has been shown to have a broad range of applications. Earlier work by the authors using ANN models to predict carbon dioxide (CO2), carbon monoxide (CO), oxides of nitrogen (NOx), and particulate matter (PM) from heavy-duty diesel engines and vehicles yielded marginal to excellent results. These ANN models can be a useful tool in inventory prediction, hybrid vehicle design optimization, and incorporated into a feedback loop of an on-board, active fuel injection management system. In this research, the ANN models were trained on continuous engine and emissions data. The engine data were used as inputs to the ANN models and consisted of engine speed, torque, and their respective first and second derivatives over a one, five, and ten second time range. The continuous emissions data were the desired output that the ANN models learned to predict through an iterative training process.