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This work is a continuation of earlier results presented by the authors. In the current investigations the biofuels hydrogenated vegetable oil (HVO) and 1-octanol are investigated as pure components and compared to EN 590 Diesel. In a final step both biofuels are blended together in an appropriate ratio to tailor the fuels properties in order to obtain an optimal fuel for a clean combustion. The results of pure HVO indicate a significant reduction in CO-, HC- and combustion noise emissions at constant NOX levels. With regard to soot emissions, at higher part loads, the aromatic free, paraffinic composition of HVO showed a significant reduction compared to EN 590 petroleum Diesel fuel. But at lower loads the high cetane number leads to shorter ignition delays and therefore, ignition under richer conditions.