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Measuring the real-world performance of emission control technologies is an important aspect in the development of advanced low-emission vehicles. In addition, data acquired from such measurements can be used to improve the accuracy of air quality predictive models. Honda has developed an on-board sampling/analysis system capable of measuring on-road emissions at ULEV levels and below. Ambient air can be analyzed simultaneously. This FTIR-based system can measure several species; this paper will focus on NMHC, NOX, and CO. Techniques were developed to address the challenges associated with acquiring accurate real-time data at concentrations below 1 ppm in an on-road vehicle. Validation studies performed with reference gases and vehicle exhaust indicate a very good correlation between the on-road analyzer system and classic bench methods for all target compounds. Dynamic studies performed by the University of California, Riverside, also show good correlation.