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The attention on emissions of two-wheelers has been poor in the past, but today in countries with a large two-wheeler population it gives a significant contribution to aggregate emissions. In this paper the results obtained on a fleet composed by 22 two-stroke motorcycles (including mopeds) are presented. Sixteen in-use mopeds and six 125 cm3 motorcycles have been tested over ECE 47 and ECE 40 driving cycles respectively. Regulated emissions (CO, HC, NOx), carbon dioxide, benzene and fuel consumption have been evaluated by fueling motorcycles with two different gasoline formulations. One gasoline was a commercial Italian leaded gasoline with 1% benzene content; the other was a lower benzene and aromatics content gasoline. Benzene emissions decreased according to benzene content of gasoline.