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In this paper, a high temporal resolution optical technique, based on the multi-wavelength UV-visible-near IR extinction spectroscopy, was applied at the exhaust of an automotive diesel engine to investigate the post-injection strategy impact on the fuel vapor. Experimental investigations were carried out using three fuels: commercial diesel (B5), a blend of 80% diesel with 20% by vol. of gasoline (G20) and a blend of 80% diesel with 20% by vol. of n-butanol (BU20). Experiments were performed at the engine speed of 2500rpm and 0.8MPa of brake mean effective pressure exploring two post-injection timings and two EGR rates. The optical diagnostic allowed evaluating, during the post-injection activation, the evolution of the fuel vapor in the engine exhaust line. The investigation was focused on the impact of post-injection strategy and fuel properties on the aptitude to produce hydrocarbon rich gaseous exhaust for the regeneration of diesel particulate trap (DPF).