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Nitrogen oxides, collectively called NOx, from diesel vehicles are considered to be accumulated by particular area of roadsides, so-called "Hot-spot," and result in harmful influence to pedestrians and residents by roadsides. Japanese regulations over emissions of diesel vehicles have been tightened year by year and adopting regulations, emissions in mode test on chassis dynamometer or engine dynamometer have reduced. In this research, it was investigated the effect of introduce of transient mode test, Japanese JE05 mode, to NOx emission in real world and to roadside NOx pollution by road test using on-board measurement system. As test vehicles, 2 ton diesel vehicle which is adopted for Long Term Regulation (steady-state mode test, Diesel 31 mode test, 1998) and 3 ton diesel vehicle adopted for New Long Term Regulation (transient mode test, Japanese JE05 mode, 2005) with on-board measurement system was used.