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Michigan Technological University has established a broad-based university-wide research initiative, termed Wood-to-Wheels (W2W), to develop and evaluate improved technologies for growing, harvesting, converting, and using woody biomass in renewable transportation fuel applications. The W2W program bridges the entire biomass development-production-consumption life cycle with research in areas including forest resources, bioprocessing, engine/vehicle systems, and sustainable decisions. The W2W chain establishes a closed cycle of carbon between the atmosphere, woody biomass, fuels, and vehicular systems that can reduce the accumulation of CO2 in the atmosphere. This paper will summarize the activities associated with the Wood-to-Wheels initiative and describe challenges and the potential benefits that are achievable.