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To better understand real fuel effects on LSPI, a matrix was developed to vary certain chemical and physical properties of gasoline. The primary focus of the study was the impact of paraffinic, olefinic, and aromatic components upon LSPI. Secondary goals of this testing were to study the impact of ethanol content and fuel volatility as defined by the T90 temperature. The LSPI rate increased with ethanol content but was insensitive to olefin content. Additionally, increased aromatic content uniformly led to increased LSPI rates. For all blends, lower T90 temperatures resulted in decreased LSPI activity. The correlation between fuel octane (as RON or MON) suggests that octane itself does not play a role; however, the sensitivity of the fuel (RON-MON) does have some correlation with LSPI. Finally, the results of this analysis show that there is no correlation between the laminar flame speed of a fuel and the LSPI rate.