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Spray characteristics of biodiesel oil was investigated as it can be applied to industrial combustion systems, including internal combustion engines. Shadowgraph methodology using Greenfield system was used to take some images of the spray and to measure droplet size. A high speed video camera was also used to take a picture of spray penetration and its angle. From the results, it shows that DME blended biodiesel oil has almost the same droplet size as conventional diesel oil, when the blended DME ratio is over 50% by weight. It is also shown that there exists optimum fuel injection pressure that has minimum droplet size when the ambient gas pressure is constant.