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A method to design a feasible multi-component fuel for fuel concentration measurements by using PLIF was developed based on thermal gravity (TG) analysis and vapor-liquid equilibrium (VLE) calculations. Acetone, toluene, and 1,2,4-trimethylbenzene were respectively chosen as tracers for the light, medium, and heavy components of gasoline. A five-component test fuel was designed for LIF measurement, which contains n -pentane (light), isooctane, n -octane (medium), n -nonane and n -decane (heavy). The TG analysis and VLE calculation were used to ensure that the fuel had volatility similar to real gasoline and that all the tracers had a good coevaporation ratio. The fully optimized results of the six-component fuel and the disadvantages of this case are discussed. The results indicated that optimization based on the six-component fuel, which included C4 compounds such as n -butane, controlled acetone's coevaporation ratio.