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Increasing biodiesel content in mineral diesel is being promoted considerably for road transportation in Europe. With positive benefits in terms of net CO2 emissions, biofuels with compatible properties to those of conventional diesel are increasingly being used in combustion engines. In comparison to standard diesel fuel, the near zero sulphur content and low levels of aromatic compounds in biodiesel fuel can have a profound effect not only on combustion characteristics but on engine-out emissions as well. This paper presents analysis of particulate matter (PM) emissions from a turbo-charged, common rail direct injection (DI) V6 Jaguar engine operating with an RME (rapeseed methyl ester) biodiesel blended with ultra low sulphur diesel (ULSD) fuel (B30 - 30% of RME by volume). Three different engine load and speed conditions were selected for the test and no modifications were made to the engine hardware or engine management system (EMS) calibration.