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The Biomass Production System (BPS) was flown on the ISS for 73 days as part of the Increment 4 mission. To obtain maximum benefit from the long mission duration, numerous manual crew procedures were incorporated into the BPS experiments. These procedures included gas sampling, root module priming, harvesting, pollination, filter cleaning, water refill, and water sampling. On-orbit crew assessments were filled out for each of these procedures to evaluate the ability of BPS to accommodate them. The assessment asked questions about each phase of an activity and solicited recommendations for improvements. Further analysis of most procedures was provided by detailed video made on-orbit and multiple post-flight crew debriefs. Most assessments indicated no need for improvements, but a number of crew suggestions will be incorporated into hardware and procedure updates.

The Advanced Animal Habitat (AAH) represents the next generation of Space Station based animal research facilities. Care has been taken to protect the ISS crew from exposure to the hazardous biological materials contained within the AAH. These materials include rat feces, urine, dander, and odor. The approach to containing biological materials relies on collecting the solid and liquid waste, providing physical barriers between the waste and the crew environment, maintaining negative pressure within the specimen environment with respect to the crew environment, and providing odor filtration of air exchanged between the specimen and crew environments. These protections will be in place during all modes of AAH operation.