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The interaction of fuel sprays and in-cylinder flow in direct-injection engines is expected to alter kinetic energy and integral length scales at least during some portions of the engine cycle. High-speed particle image velocimetry was implemented in an optical four-valve, pent-roof spark-ignition direct-injection single-cylinder engine to quantify this effect. Non-firing motored engine tests were performed at 1300 RPM with and without fuel injection. Two fuel injection timings were investigated: injection in early intake stroke represents quasi-homogenous engine condition; and injection in mid compression stroke mimics the stratified combustion strategy. Two-dimensional crank angle resolved velocity fields were measured to examine the kinetic energy and integral length scale through critical portions of the engine cycle. Reynolds decomposition was applied on the obtained engine flow fields to extract the fluctuations as an indicator for the turbulent flow.