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The Closed Ecology Experiment Facilities (CEEF) were installed to collect data for estimation of transfer of radionuclides from atmosphere to humans in the ecosystem. The first target among the radio-nuclides is 14C. In order to validate function of material circulation in an experimental system constructed in the CEEF, circulation of air constituents, water and materials in waste was demonstrated connecting the Closed Plant Experiment Facility (CPEF) and the Closed Animal and Human habitation Experiment Facility (CAHEF) of the CEEF, since 2005 to 2007. The CPEF has a Plant Cultivation Module (PCM), which comprises of three plant chambers illuminated solely by artificial lighting, one plant chamber illuminated by both natural and artificial lighting, a space for preparation, and an airlock, and a physical/chemical material circulation system.

Rating of daily amounts of CO2 and O2 exchange of crops, animals and humans to be involved in the long-term habitation experiments using the Closed Ecology Experiment Facilities (CEEF) were carried out. Daily amounts of the CO2-absorption and O2-generation of crops including rice, soybeans and other 27 vegetables were estimated from data obtained from a sequential crop cultivation experiment conducted from August to December of FY2001. Daily amounts of O2-consumption and CO2-expiration of two female Shiba goats to be involved were estimated based on gas exchange determination conducted in FY2002. Daily amounts of CO2-expiration and O2-consumption of two persons to be involved were estimated based on correlation between respiration rate and heart rate, heart rate data during the simulated habitation in the CEEF and a tentative work schedule.

In the Closed Ecology Experiment Facilities (CEEF), with integrating the Closed Plantation Experiment Facilities (CPEF) and the Closed Animal Breading & Habitation Facilities (CABHF), closed habitation experiments without material exchange with the outside will be conducted after the 2005 fiscal year. Cultivation experiments of about 30 crops and the integrating test of the material circulation system required for the closed habitation experiments have been performed since 2000 fiscal year. Using data reported in these experiments, material circulation in CEEF is simulated based on the recent operation strategy, and the storage capacity needed for the buffer of an air processing subsystem was estimated. In order for two humans to dwell over 120 days, the storage capacities of the carbon dioxide tank, the oxygen tank, and the waste gas tank in CPEF, and the carbon dioxide tank and the oxygen tank in CABHF are 820 g, 2830 g, 4425 g, 1780 g, and 1792 g, respectively.

A simulation of an open mode system experiment was run using the same experimental conditions as an integration test conducted from September 1999 to February 2000 using the Closed Plant Experiment Facility at the Institute for Environmental Sciences in order to evaluate the operation of closed mode system to be conducted in future. Operation of the open mode system experiment required a supply of water and carbon dioxide from the outside, and the discharge of nutrient waste water and oxygen. The present simulation verified the feasibility of using non-integrated wet-oxidation processor, nutrient synthesis unit and nutrient waste water processor connected within a closed mode system, and it was confirmed that sufficient material circulation could be achieved when rice and soybeans were divided into six beds with different growing stages to facilitate control of the nutrient solution.

Many research activities relating the environment control and life support systems to be used in space have been conducted and continued in Japan since 1982 in order to obtain his own manned flight technologies for future Japanese missions. Research and development activities are able to be divided in two phases according mission scheduling such as JEM development to be used in International Space Station now going on and the future Japanese space infrastructures, for examples, Japanese Space Station and Lunar Base. Temperature, humidity, total atmospheric pressure and partial pressure of oxygen and carbon dioxide, necessary for all living things, are to be controlled by the environment control function.

As a CO2 removing and recovering technology, the solid amine utilized as a vacuum desorbing type was developed. The evaluation experiments to measure fundamental characteristics of this material was conducted using the dry air and as the results, the high adsorbing rate, 10 wt% for short period and 7 wt% for long period was obtained and also 7 wt% of adsorbing rate can be expected for the operation of desorption temperature of 80 °C. High accuracy thermobalance device so called thermogravimetric analyzer was utilized to measure adsorption and desorption values of CO2.

In the closed environments such as manned space station, it is necessary to remove contaminant gas to keep a suitable environment. Removal of gaseous contaminants generated from crew, animals, and plants is important function to keep the environment below the allowable level in the Closed Ecology Experiment Facilities (abbreviated as CEEF). CEEF consist of three modules for habitat, animal and plant, the supporting facilities for each module and a plant cultivation facility. CEEF are scheduled to be constructed from 1994 in Aomori Prefecture, northern part of Japan. For designing Trace Contaminant Control Assembly (TCCA) for CEEF, the following six (6) trace contaminants have been selected as major contaminant gas in CEEF; Ammonia (NH3) Methane (CH4) Ethylene (C2H4) Carbon Monoxide (CO) Nitrogen Dioxide (NO2) Sulfur Dioxide (SO2) Ethylene is well-known as an aggressive contaminant to plant growth and maturity.

Compared with other applicable processes such as incineration, the catalytic wet oxidation process is considered to be the most practically applicable waste treatment process for the CELSS. In this report, the quantity of carbon monoxide generated in the wet oxidation process is identified and a measure for carbon monoxide minimization is discussed. As a result of a bench test, it became apparent that a non-negligible quantity of carbon monoxide could be generated in the catalytic wet oxidation process. However, it can be expected that this CO content will be reduced to a safe level by applying the wet oxidation process catalyst reactor to CO oxidation.

The basal metabolism of the Candidate Animal is mainly on energy metabolism that was estimated for future animal breeding in CEEF as preliminary research. The amounts of gas exchange in the respiration and heat production of the Shiba goat (native Japanese goat) were analyzed to predict energy and material flow of the animal breeding system in the Closed Ecology Experiment Facilities (CEEF). Experimental animals were fed Timothy hay or inedible parts of rice cultivated in CEEF. The feces and urine were collected during the 7-day metabolism measurement period after a 2-week preliminary breeding period. The O2 consumption, CO2 production, and CH4 production were measured by a mass spectrometric respiration gas analysis system on the 7th day of the metabolism measurement period. Heat production was also obtained from these data. O2 consumption, CO2 production and CH4 production were 100.3 - 153.8 L, 127.2 - 174.0 L and 5.7 - 10.8 L per day (at 0°C, 0.101MPa), respectively.