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This paper deals with the combustion characteristics and exhaust emissions of a diesel engine fuelled with conventional diesel fuel and a biodiesel blend, in particular a 20% v/v concentration of rapeseed methyl ester (RME) mixed with diesel fuel. The investigation was carried out on a prototype three-cylinder engine with 1000 cc of displacement for quadricycle applications. The engine is equipped with a direct common-rail injection system that reaches a maximum pressure of 1400 bar. The engine was designed to comply with Euro 4 and BS IV exhaust emission regulations without a diesel particulate filter. Both in-cylinder pressure and rate of heat release traces were analyzed at different engine speeds and loads. Gaseous emissions were measured at the exhaust. A smoke meter was used to measure the particulate matter concentration. The sizing and the counting of the particles were performed by means of an engine exhaust particle sizer spectrometer.