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To study the effect of different injection timings on the charge inhomogeneity, planar laser-induced fluorescence (PLIF) was applied to an operating engine. Quantitative images of the fuel distribution within the engine were obtained. Since the fuel used, iso-octane, does not fluoresce, a dopant was required. Three-pentanone was found to have vapour pressure characteristics similar to those of iso-octane as well as low absorption and suitable spectral properties. A worst case estimation of the total accuracy from the PLIF images gives a maximum error of 0.03 in equivalence ratio. The results show that an early injection timing gives a higher degree of charge inhomogeneity close to the spark plug. It is also shown that charge inhomogeneity gives a more unstable engine operation. A correlation was noted between the combustion on a cycle to cycle basis and the average fuel concentration within a circular area close to the spark plug center.