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For applications of planar laser induced fluorescence (PLIF) to measure the fuel or equivalence ratio distributions in internal combustion (IC) engines it is typically assumed that the addition of a fluorescence tracer to a base fuel does not alter the combustion performance. We have investigated the impact on combustion performance through the addition of various amounts of 3-pentanone or toluene to iso-octane fuel. Correlations between equivalence ratio for a range of fuel/tracer mixtures and engine parameters, like peak pressure, location of peak pressure, indicated mean effective pressure (IMEP), and peak burn rate are discussed for data obtained in a spark-ignition direct-injection (SIDI) gasoline engine operated with near homogeneous charge. For typical tracer concentrations the impact on combustion performance is mostly negligible.