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A biodiesel fuel, obtained from Jatropha seed in China, was tested in a direct injection, high pressure common-rail diesel engine for passenger cars. Effects of biodiesel on particle number and size distribution of the diesel engine are studied using an Engine Exhaust Particle Sizer (EEPS). Base petroleum diesel fuel, 10% and 20% v/v biodiesel blends with the base petroleum diesel fuel, the biodiesel fuel (B0, B10, B20 and B100 fuels) were tested without engine modification. For all test fuels, the particle number and size distribution show unimodal or bimodal log-normal distribution, with a nucleation mode peak value in 6.04nm to 10.8nm particle diameter, and with an accumulation mode peak value in 39.2nm to 60.4nm particle diameter.