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The measurement of the particle number (PN) concentration of non-volatile particles ≻23 nm was introduced in the light-duty vehicles regulation; the heavy-duty regulation followed. Based on the findings of the Particle Measurement Program (PMP), heavy-duty inter-laboratory exercise, the PN concentration measurement can be conducted either from the full dilution tunnel with constant volume sampling (CVS) or from the partial flow dilution system (PFDS). However, there are no other studies that investigate whether the PN results from the two systems are equivalent. In addition, even the PMP study never investigated the uncertainty that is introduced at the final result from the extraction of a flow by a PN system from the PFDS. In this work we investigate the uncertainty for the three possible cases, i.e., considering a constant extracted flow from the PFDS, sending a signal with 1 Hz frequency to the PFDS, or feeding back the extracted flow to the PFDS.