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Fatigue defect and failure of rubber element widely used in mechanical systems could seriously affect the safety and reliability of systems in practical operation. Because rubber element is considered as hyperelastic material, traditional σ - N curve which is usually used in metal material for fatigue life analysis can not be used here. The fatigue life of rubber bushing in automobile engine cradle was analyzed by using the energy method. The Yeoh model coefficients were given by tensile test of natural rubber, and the estimating formula for fatigue life of natural rubber was obtained by finite element calculation and fatigue test. Maximum strain energy density was treated as the parameter of fatigue damage, then the rubber bushing fatigue life was calculated by the estimating formula. The results were verified by test of rubber bushing, which indicated that the model mentioned in this paper is accuracy enough.