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In 1990, Roy Douglas developed an analytical method to calculate the global air-to-fuel ratio of a two-stroke engine from exhaust gas emissions. While this method has considerable application to two-stroke engines, it does not permit the calculation of air-to-fuel ratios for oxygenated fuels. This study proposed modifications to the Roy Douglas method such that it can be applied to oxygenated fuels. The ISO #16183 standard, the modified Spindt method, and the Brettschneider method were used to evaluate the modifications to the Roy Douglas method. In addition, a trapped air-to-fuel ratio, appropriate for two-stroke engines, was also modified to incorporate oxygenated fuels. To validate the modified calculation method, tests were performed using a two-stroke carbureted and two-stroke direct injected marine outboard engine over a five-mode marine test cycle running indolene and low level blends of ethanol and iso-butanol fuels.