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The methyl esters of vegetable oils, known as biodiesel are becoming increasingly popular because of their low environmental impact and potential as a green alternative fuel for diesel engine and they would not require significant modification of existing engine hardware. Methyl ester of rice bran oil (ROME) is derived through transesterification process. Previous research has shown that ROME has comparable performance, lower bsfc in comparison to diesel. There was reduction in the emissions of CO, HC, and smoke but NOx emissions increased. Experimental investigations have been carried out to examine the combustion characteristics in a direct injection transportation diesel engine running with diesel, and 20% blend of rice bran methyl ester with diesel.