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Simultaneous formaldehyde and OH PLIF have been applied in a direct-injected HCCI engine. The engine is a 0.5 l single-cylinder optical engine equipped with EGR system. PLIF measurements were performed with the engine run with two different fuels of low and high volatility, respectively. Different ratios of EGR were also examined. The aim of the study was to investigate how fuels with different volatility and EGR affect the HCCI combustion and measurements were performed for early and late injection timings. Measurements are presented for different injection timings showing formaldehyde and OH from start of injection until late in the expansion stroke. Also, formaldehyde distributions obtained from after the low temperature regime and before the high temperature regime are studied for different tuning of the start of injection from 300CAD to 20CAD before top dead center.