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Environmental concerns have increased significantly world over in the past decade. Regulatory agencies are becoming increasingly concerned with particulate emissions as the health and environmental effects are getting understood better due to rapid development in instrumentation. Biodiesel is one of the most promising alternative diesel fuels, which is getting global acceptability among the automotive/ engine manufactures as well as users due to numerous benefits it offers over the conventional diesel. While much of literature is available on particulate emitted by diesel fuelled engine, little is known by particulate emissions from biodiesel fuelled compression ignition (CI) engine. This study concentrates on the characterization of particulate emissions from mineral diesel vis-à-vis biodiesel (B100) and its optimum blend (20%, B20) with mineral diesel.